Adipolysis Assay Kit (EAPL-200)

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems' adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.

• For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
• Method: Colorimetric (570nm), or fluorometric (530/585nm) assay. A spectrophotometer or plate reader is required, but not provided.
• Samples: Cell culture media. Species: All. Detects 0.92 ug/mL
• Procedure: 20 min. Size: 200 tests per kit